14.10 Homocysteine

14.10.1 Global tests

Type III ANOVA on main and interaction effects, and LM model summaries.

PRL (ng/ml)
Chisq Df Pr(>Chisq)
(Intercept) 235.6994335 1 <0.0001 ***
Homocysteine 7.3576733 1 0.007 **
Study 0.1255518 1 0.7 ns
GA_samp 154.3594749 1 <0.0001 ***
Homocysteine:Study 0.2597121 1 0.6 ns
hPL (μg/ml)
Chisq Df Pr(>Chisq)
(Intercept) 40.5521082 1 <0.0001 ***
Homocysteine 0.1961999 1 0.7 ns
Study 7.1515275 1 0.007 **
GA_samp 2393.3046784 1 <0.0001 ***
Homocysteine:Study 1.2745922 1 0.3 ns
GH2 (ng/ml)
Chisq Df Pr(>Chisq)
(Intercept) 330.0154029 1 <0.0001 ***
Homocysteine 0.5471963 1 0.5 ns
Study 0.5310165 1 0.5 ns
GA_samp 1048.0376763 1 <0.0001 ***
Homocysteine:Study 0.4835906 1 0.5 ns
Insulin (uU/ml)
model term df1 df2 F.ratio Chisq p.value
1 Homocysteine 1 Inf 23.350 23.350 <0.0001 ***
3 Study 1 Inf 6.563 6.563 0.01 *
4 GA_samp 1 Inf 0.436 0.436 0.5 ns
2 Homocysteine:Study 1 Inf 2.155 2.155 0.1 ns
HOMA-IR
model term df1 df2 F.ratio Chisq p.value
1 Homocysteine 1 Inf 22.244 22.244 <0.0001 ***
3 Study 1 Inf 11.487 11.487 0.0007 ***
4 GA_samp 1 Inf 0.170 0.170 0.7 ns
2 Homocysteine:Study 1 Inf 2.708 2.708 0.10 ns
Glucose (mmol/L)
model term df1 df2 F.ratio Chisq p.value
1 Homocysteine 1 Inf 6.334 6.334 0.01 *
3 Study 1 Inf 23.093 23.093 <0.0001 ***
4 GA_samp 1 Inf 0.681 0.681 0.4 ns
2 Homocysteine:Study 1 Inf 2.659 2.659 0.1 ns

14.10.2 Model summary

Ratio of Geometric means (95% CI)

PRL (ng/ml)
Var Effect P
(Intercept) 21.93 (14.97, 32.12) <0.0001
Homocysteine 0.94 (0.92, 0.97) <0.0001
StudySTOP 0.99 (0.89, 1.09) 0.80
GA_samp 1.14 (1.12, 1.17) <0.0001
hPL (μg/ml)
Var Effect P
(Intercept) 2.00 (1.64, 2.44) <0.0001
Homocysteine 0.99 (0.97, 1.00) 0.05
StudySTOP 1.12 (1.06, 1.18) <0.0001
GA_samp 1.34 (1.32, 1.35) <0.0001
GH2 (ng/ml)
Var Effect P
(Intercept) 0.07 (0.06, 0.10) <0.0001
Homocysteine 1.00 (0.98, 1.02) 0.74
StudySTOP 0.99 (0.92, 1.07) 0.81
GA_samp 1.30 (1.28, 1.32) <0.0001
Insulin (uU/ml)
Var Effect P
(Intercept) 20.83 (12.54, 34.60) <0.0001
Homocysteine 0.92 (0.88, 0.95) <0.0001
StudySTOP 0.79 (0.69, 0.92) 0.001
GA_samp 1.01 (0.98, 1.04) 0.65
HOMA-IR
Var Effect P
(Intercept) 5.43 (3.18, 9.27) <0.0001
Homocysteine 0.91 (0.88, 0.95) <0.0001
StudySTOP 0.68 (0.59, 0.80) <0.0001
GA_samp 1.00 (0.97, 1.03) 0.87
Glucose (mmol/L)
Var Effect P
(Intercept) 6.04 (5.47, 6.68) <0.0001
Homocysteine 0.99 (0.98, 1.00) 0.01
StudySTOP 0.86 (0.84, 0.88) <0.0001
GA_samp 1.00 (0.99, 1.00) 0.26

14.10.3 Estimated means

Estimated marginal means (or adjusted means) adjusted for GA at sampling.

Results are averaged over the levels of Homocysteine concentration (μM) and may be misleading when interaction term is involved.

PRL (ng/ml)
Study response SE df lower.CL upper.CL
SCOPE 96.86703 7.187378 83.56245 83.57767 112.2695
STOP 95.57348 6.784953 70.13543 82.95606 110.1100
hPL (μg/ml)
Study response SE df lower.CL upper.CL
SCOPE 88.18762 2.315622 144.6046 83.72752 92.88531
STOP 98.66412 2.376767 102.6966 94.06104 103.49247
GH2 (ng/ml)
Study response SE df lower.CL upper.CL
SCOPE 2.330353 0.0640586 389.1314 2.207752 2.459763
STOP 2.309239 0.0531721 222.5795 2.206796 2.416438
Insulin (uU/ml)
Study response SE df asymp.LCL asymp.UCL
SCOPE 14.73718 0.7077907 Inf 13.41323 16.19182
STOP 11.69889 0.4205725 Inf 10.90295 12.55293
HOMA-IR
Study response SE df asymp.LCL asymp.UCL
SCOPE 3.578746 0.1827232 Inf 3.237951 3.955409
STOP 2.447504 0.0928142 Inf 2.272187 2.636348
Glucose (mmol/L)
Study response SE df asymp.LCL asymp.UCL
SCOPE 5.481045 0.0482272 Inf 5.387332 5.576389
STOP 4.715530 0.0327959 Inf 4.651688 4.780249

14.10.4 Comparisons

Effect for every 1 unit increase in Homocysteine concentration (μM).
Hormone Contrast Study Ratio of Geometric means (95% CI) P-value
PRL (ng/ml) Homocysteine5.5 / Homocysteine4.5 SCOPE 0.94 (0.92, 0.97) <0.0001
PRL (ng/ml) Homocysteine5.5 / Homocysteine4.5 STOP 0.94 (0.92, 0.97) <0.0001
hPL (μg/ml) Homocysteine5.5 / Homocysteine4.5 SCOPE 0.99 (0.97, 1.00) 0.05
hPL (μg/ml) Homocysteine5.5 / Homocysteine4.5 STOP 0.99 (0.97, 1.00) 0.05
GH2 (ng/ml) Homocysteine5.5 / Homocysteine4.5 SCOPE 1.00 (0.98, 1.02) 0.7
GH2 (ng/ml) Homocysteine5.5 / Homocysteine4.5 STOP 1.00 (0.98, 1.02) 0.7
Insulin (uU/ml) Homocysteine5.5 / Homocysteine4.5 SCOPE 0.92 (0.88, 0.95) <0.0001
Insulin (uU/ml) Homocysteine5.5 / Homocysteine4.5 STOP 0.92 (0.88, 0.95) <0.0001
HOMA-IR Homocysteine5.5 / Homocysteine4.5 SCOPE 0.91 (0.88, 0.95) <0.0001
HOMA-IR Homocysteine5.5 / Homocysteine4.5 STOP 0.91 (0.88, 0.95) <0.0001
Glucose (mmol/L) Homocysteine5.5 / Homocysteine4.5 SCOPE 0.99 (0.98, 1.00) 0.01
Glucose (mmol/L) Homocysteine5.5 / Homocysteine4.5 STOP 0.99 (0.98, 1.00) 0.01

14.10.5 Diagnostics

Model diagnositcs.

Diagnositcs for PRL (ng/ml)

Diagnositcs for hPL (μg/ml)

Diagnositcs for GH2 (ng/ml)

Diagnositcs for Insulin (uU/ml)

Diagnositcs for HOMA-IR

Diagnositcs for Glucose (mmol/L)

14.10.6 Plots